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. 2014 Oct 30;13:147. doi: 10.1186/s12934-014-0147-7

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© Dato et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Growth of wild type and sam2Δ leucine nutritionally complemented strains in the absence and presence of lactic acid. Yeast cells were grown in shake flasks in minimal (YNB) medium with 2% w/v glucose and 50 mg/L of the necessary nutritional supplements at initial pH 3, without (panel A) or with (panel B) 34 and 38 g/l of lactic acid for CEN.PK (left panels) and BY (right panels), respectively. Growth was determined as OD at 660 nm. Light grey squares: parental wild type strains (CEN.PK 102-3A, BY4741). White triangles: sam2Δ cells (CEN.PK 102-3A sam2Δ, BY4741 sam2Δ).