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. 2014 Oct 30;13:147. doi: 10.1186/s12934-014-0147-7

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© Dato et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Batch lactic acid production in wt and sam2Δ strains. Fermentation profiles for CEN.PK m850 (filled symbols) and CEN.PK m850 sam2Δ (open symbols) pre-grown in shake flasks and then transferred in new flasks in minimal medium containing 90 g/L of glucose for the lactic acid production phase. (A) Biomass formation (OD at 660 nm). (B) Residual glucose (diamonds) and produced lactic acid (triangles). (C) Cellular viability was determined by PI staining followed by flow cytometry. (D) Culture medium pH. Panel A and B report the mean and SD for three independent experiments (*p ≤ 0.01; Student’s t-test; for supplementary details see Additional file 2: Table S1); Panel C and D report data from a single representative experiment.