Table 2.
Yeast strains used and created in this study
| Strain | Relevant genotype | Plasmid | Reference |
|---|---|---|---|
| CEN.PK 113-11C | MATa, ura3-52, his3-Δ1 | - | P. Kotter1 |
| CEN.PK 102-3A | MATa, ura3-52, leu2-3,112 | - | P. Kotter1 |
| CEN.PK [pTEF-L] | CEN.PK 102-3A | pTEF-L, multicopy | This work |
| (ScTEF1, LEU2) | |||
| CEN.PK [pTEF-L-SAM2] | CEN.PK 102-3A | pTEF-L-SAM2 | This work |
| (ScTEF1, ScSAM2, LEU2) | |||
| CEN.PK sam2Δ | CEN.PK 102-3A sam2::KanMX4 | - | This work |
| CEN.PK sam2Δ [pTEF-L] | CEN.PK 102-3A sam2Δ | pTEF-L | This work |
| (ScTEF1, LEU2) | |||
| CEN.PK SAM2GFP | CEN.PK 113-11C | - | This work |
| SAM2:: SAM2GFP-HIS3 | |||
| CEN.PK m850 | MATa, pdc1(-6,-2)::loxP, | YEpLpLDH | [18] |
| pdc5(-6,-2)::loxP, pdc6 (-6,-2)::loxP, ura3-52, acid tolerant | (ScTPI, LpLDH, URA3) | ||
| CEN.PK m850 sam2Δ | CEN.PKm850 sam2::KanMX4 | YEpLpLDH | This work |
| (ScTPI, LpLDH, URA3) | |||
| BY4741 | MATa, his3-Δ1, leu2-Δ0, met15-Δ0, ura3-Δ0 | - | EUROSCARF2 |
| BY4741[pTEF-L] | BY4741 | pTEF-L | This work |
| (ScTEF1, LEU2) | |||
| BY4741[pTEF-L-SAM2] | BY4741 | pTEF-L-SAM2 | This work |
| (ScTEF1, ScSAM2, LEU2) | |||
| BY4741 sam2Δ | BY4741 sam2::kanMX4 | - | This work |
| BY4741 sam2Δ [pTEF-L] | BY4741 sam2Δ | pTEF-L | This work |
| (ScTEF1, LEU2) | |||
| BY4741 SAM2GFP | BY4741 SAM2:: SAM2GFP-HIS3 | - | This work |
1Institut fur Mikrobiologie der Johann Wolfgang Goethe Universitat, Frankfurt, Germany.