Table 3.

Performance of the wild-type P . putida KT2440 and the streamlined strain EM383 as a host for recombinant DNA

P. putida strain	Plasmid transfer efficiency (transformants per 1 × 10 9 cells)
	Mating a	Electroporation b
KT2440	(5 ± 1) × 106	5,483 ± 3,060
EM383	(18 ± 5) × 106	1,642 ± 822

^aThe plasmid transfer efficiency by tri-parental mating was calculated using vector pSEVA221, and the figures represent the mean value and SD of three biological replicates.

^bThe plasmid transfer efficiency by electroporation was calculated using vector pSEVA251, and the figures represent the mean value and SD of three biological replicates when using 1 ng of vector.