Table 3.
P. putida strain | Plasmid transfer efficiency (transformants per 1 × 10 9 cells) | |
---|---|---|
Mating a | Electroporation b | |
KT2440 | (5 ± 1) × 106 | 5,483 ± 3,060 |
EM383 | (18 ± 5) × 106 | 1,642 ± 822 |
aThe plasmid transfer efficiency by tri-parental mating was calculated using vector pSEVA221, and the figures represent the mean value and SD of three biological replicates.
bThe plasmid transfer efficiency by electroporation was calculated using vector pSEVA251, and the figures represent the mean value and SD of three biological replicates when using 1 ng of vector.