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. 2014 Oct 1;25(19):2919–2933. doi: 10.1091/mbc.E14-02-0735

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© 2014 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Cby1 facilitates ciliary recruitment of Arl13b. (A) Cby1^+/+ or Cby1^−/− MEFs were serum starved for 48 h, fixed, and stained for acetylated α-tubulin (Ac-tub, red), Arl13b (green), and DNA (DAPI, blue). Scale bar, 5 μm. (B, C) Quantification of ciliary Arl13b and acetylated tubulin levels in Cby1^+/+ or Cby1^−/− MEFs. Results shown are the mean of three independent experiments ± SEM (>100 cells/experiment, *p < 0.05). (D) Cby1^−/− MEFs were transduced with lentivirus expressing GFP or GFP-Cby1, serum-starved for 48 h, fixed, and stained for GFP (green), Arl13b (red), and DNA (DAPI, blue). Scale bar, 5 μm. (E) Ciliary Arl13b intensity was quantified and displayed as a Tukey boxplot. Results from duplicate independent experiments, >30 observations/experiment, ***p < 0.001 by Mann–Whitney U test.