Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 1;25(19):2956–2969. doi: 10.1091/mbc.E14-04-0894

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Yashiro et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — Regulators of myosin II downstream of Rho1 affect AJ remodeling and Rab11 staining. Confocal immunofluorescence localization of DE-cadherin (DE-cad, red; A, A′) and Rab11 (A′′) in AJ region of GFP-labeled PECs expressing Rok RNAi. Confocal immunofluorescence localization of DE-cad (red; B, B′, C), pMLC (B′′), and Rab11 (C′) in AJ region of GFP-labeled PECs expressing the catalytic domain of Rok (Rok-CAT). White arrowheads denote wild-type PECs, and yellow arrowheads denote mutant PECs. Confocal immunofluorescence localization of DE-cad (red; D, D′) and Rab11 (D′′) in AJ region of GFP-labeled Rho1^72F (Rho1 LoF) MARCM clones coexpressing Rok-CAT. Heat maps of Rab11 immunofluorescence in merged confocal slices through the AJ region in wild type (E) and Rho1^72F MARCM clones coexpressing Rok-CAT (E′). Confocal immunofluorescence localization of DE-cad (red; F, F′, G), pMLC (F′′), and Rab11 (G′) in AJ region of PECs coexpressing Rho1 RNAi and wild-type Zip (Zip). Representative heat maps of Rab11 immunofluorescence in merged confocal slices in AJ region of wild-type PECs (H) and PECs coexpressing Rho1 RNAi and Zip (H′). Confocal immunofluorescence localization of DE-cad (red; I, I′) and pMLC (I′′) in AJ region of GFP-labeled PECs coexpressing Rho1 RNAi and MBS RNAi. White arrowheads and asterisks denote wild-type PECs, and yellow arrowheads and asterisks denote mutant PECs. Pointed yellow arrowheads denote restored AJs. Confocal immunofluorescence localization of YFP-Rab5 (green; J, J′), Rab11 (red; J, J′′), and pMLC (blue; J, J′′′) in wild-type PECs ubiquitously expressing YFP-Rab5 (GMR-YFP-Rab5). Yellow arrowheads denote vesicles that colocalize YFP-Rab5, Rab11, and pMLC (magnified in inset), quantified as percentage Rab5- and Rab11-positive vesicles that also localize RLC-GFP. Images were compiled as a sum of multiple confocal slices within the region where AJs were present. White scale bars (lower right corner), 10 μm.