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. 2014 Oct 1;25(19):2970–2983. doi: 10.1091/mbc.E13-12-0729

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© 2014 Zhang and Oliferenko. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — The mitotic SPBs are compromised in recruiting the γ-tubulin complex in cut11-6 tts1Δ cells. (A) Time-lapse maximum z-projection images of spinning disk confocal stacks of cells with indicated genetic backgrounds coexpressing GFP-Atb2, Pcp1-mCherry, and the nucleoplasm marker protein GST-NLS-mCherry. An aster-like short spindle in early mitotic tts1Δ cells is denoted by an arrow. (B, C) Time-lapse maximum z-projection images of spinning disk confocal stacks of cells with indicated genetic backgrounds coexpressing Pcp1-mCherry and Alp4-GFP (B) or Alp6-GFP (C). Cells were grown and imaged at 24°C, the permissive temperature for cut11-6 mutant. The elapsed time is shown in minutes. Scale bars, 5 μm.