TABLE 1:
Experimental hydrodynamic radii, Svedberg coefficients, and calculated molecular weight and Smax/Sexp values.
| Rh (nm) | Sexp (S) | Smax (S) | MWexpect (kDa) | MWcalc (kDa) | Shape factor | |
|---|---|---|---|---|---|---|
| Ovalbumin | 3.1 | 3.6 | 4.5 | 43 | 46 | 1.25 |
| Albumin | 3.6 | 4.6 | 5.9 | 66 | 68 | 1.28 |
| Catalase | 5.2 | 11.3 | 13.6 | 244 | 247 | 1.20 |
| Thyroglobulin | 8.5 | 19 | 27.4 | 660 | 679 | 1.44 |
| SPD-5 | 9.5 | 4.0 | 9.5 | 135 | 160 | 2.36 |
| RSA-2 | 6.8 | 5.6 | 8.1 | 107 | 160 | 1.44 |
| RSA-1 | 6.9 | 5.7 | 4.6 | 47 | 163 | 0.81 |
| SPD-2 | 6.0 | 3.8 | 7.3 | 92 | 95 | 1.94 |
| RSA-1/RSA-2 | 6.8 | 5.6 | 10.3 | 153 | 160 | 1.80 |
Rh, hydrodynamic radius; Sexp, Svedberg value determined in experiment; Smax, maximum Svedberg value estimated for a spherical particle of a given molecular weight; MWexpect, molecular weight estimated from sequence; MWcalc, molecular weight as calculated from Rh and Sexp; Shape factor, ratio Smax/Sexp, which provides information about the shape of a molecule (Erickson et al., 2009).