TABLE 2:
D (μm2/s) | τblinking (μs) | Fraction τ (%) | α | n | χ2corr of fit | |
---|---|---|---|---|---|---|
GFP::SPD-2 | 2.7 ± 0.4 | 41 ± 45 | 10 ± 4 | 0.75 ± 0.05 | 5 ± 1 | 2.02 ± 0.35 |
LAP::RSA-1 | 2.0 ± 0.4 | 38 ± 17 | 11 ± 2 | 0.65 ± 0.06 | 19 ± 6 | 2.58 ± 0.56 |
D, diffusion coefficient; τblinking, triplet blinking decay time; fraction τ, percentage of molecules predicted to be in blinking state; α, anomalous α to correct for subdiffusive or superdiffusive behavior of particles; n, number of particles calculated to be in the focal volume at any given time; χ2corr, corrected χ2 as a measure for the goodness of the fit.