FIGURE 5:

Role of novel KLC-binding regions of JIP1b in efficient anterograde transport of APP cargo. APP-EGFP and the indicated JIP1b mutants were expressed in primary cultured neurons from JIP1-KO mice (A–D). Data are presented as in Figure 1. The transport of APP cargo is shown. Position of average velocity is indicated with arrows. See Supplemental Movie S3A for A, S3B for B, S3C for C, and S3D for D. Scale bar, 5 μm. The ratios of anterograde (A), retrograde (R), and stationary (S) vesicles are indicated in the insets at the right. Statistical analysis for direction of movement vesicles is summarized in Table 1.