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. 2014 Nov 5;25(22):3619–3629. doi: 10.1091/mbc.E14-06-1127

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© 2014 Backlund et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — (A) Behavior of the DH-PSF as a function of axial defocus (z). (B) Fluorescence images from the green (top) and red (bottom) channels at 10-s intervals of one example track pair. Scale bar, 1 μm. (C) The 2D projection of trajectories from B overlaid on white light image of whole cell. Scale bar, 1 μm.