FIGURE 7:

Graphs showing the effect of varying NTR distribution along the length of an mRNA on nuclear basket and central channel residence times for successful export events (error bars represent 1 SD). Note that all configurations used the baseline NXF1 to FG Nup affinity of 100 μM. “NTR on ½” and “NTR on ¾” represent configurations where transport receptors were placed on the terminal one-half and three-fourths length of the mRNA, respectively, with the same spacing as used in the baseline configuration for a total of five transport receptors in the one-half configuration and seven transport receptors in the three-fourths configuration. The graph on the left captures observations recorded with a double tag (5′ and 3′ end) system, and the graph on the right captures those recorded with a single tag.