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. 2014 Nov 5;25(22):3672–3685. doi: 10.1091/mbc.E14-06-1107

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© 2014 Wu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — STIM1 and Orai1 puncta fluorescence is stable over time. HEK cells expressing moderate levels of mCh-STIM1 (A and B) or mCh-myc-Orai1(C and D) together with PAGFP-labeled partner protein were store depleted with 1 μM TG in 0 Ca²⁺ Ringer's and imaged by confocal microscopy over time. Regions of interest marking selected puncta are superimposed on average images of 100-frame movies for mCh-STIM1 (A) and mCh-myc-Orai1 (C). Mean fluorescence vs. time (corrected for photobleaching) of the selected mCh-STIM1 (B) and mCh-myc-Orai1 (D) puncta shows stable puncta fluorescence over >5 min.