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. 2014 Jul 8;5(4):86. doi: 10.1186/scrt475

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Copyright © 2014 Mienaltowski et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Progenitor-seeded fibrin gels form tendon-like structures. (A) Stem/progenitor cells are seeded within fibrinogen/thrombin; at Day 2, a distinct gel bridges the two silk sutures. (B) By Day 7, the fibrin within the structure has commenced contracting. (C) By Day 14, the construct has contracted into a long, narrow structure in which tension is applied uniaxially. Representative images are from tendon proper-derived stem/progenitor-cell-seeded constructs (Bar: 200 μm).