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. 2014 Nov 13;10(11):e1004715. doi: 10.1371/journal.pgen.1004715

Figure 1. Synaptic vesicle mistargeting and transport defects in the gm379 mutant.

Figure 1

(A) A schematic diagram of the C. elegans ALM and PLM neurons and their synapses. The “+” and “−“ signs indicate the dominant microtubule orientation in the anterior ALM and PLM processes. (B-G, B′-G′) Synaptic vesicles in live animals were visualized and quantified with jsIs821(Pmec-7::GFP::RAB-3) (B-E, B′-E′) from the nerve ring synapses (B, B′), ALM soma (C, C′), PLM synapses in the ventral nerve cord (D, D′), PLM soma (E, E′, asterisks) and the PLM posterior process (E, E′). SV mistargeting was independently verified with jsIs37(Pmec-7::SNB-1::GFP) (F, F′) and jsIs219(Psng-1::SNG-1::GFP) (G, G′). Arrows, mistargeted SVs. Scale bar  = 5 µm. (H) SV abundance measured by GFP::RAB-3 quantification (mean ± S.E.M.) in respective locations of the touch neuron circuit. N = 10 animals for each genotype at each time point. (I) Percentage of animals with SV mistargeting. (J) Kymograph of SV in the PLM posterior process of the mutant.