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. 2014 Nov 13;10(11):e1004715. doi: 10.1371/journal.pgen.1004715

Figure 7. Negative charges of the EEGE cluster in the MEC-12 H12 helix instruct synaptic vesicle targeting.

Figure 7

(A) Effects of G416 substitution on SV targeting in the PLM neuron. Expression of respective MEC-12 variants in the mec-12(e1607) was indicated by the cell-autonomous marker Pdpy-30::NLS::dsRed. SVs were labeled by jsIs821(Pmec-7::GFP::RAB-3). Arrows, mistargeted SVs. Asterisks, PLM soma. Scale bar  = 5 µm. (B) Quantification of SV mistargeting in animals expressing MEC-12 variants. Array loss, transgenic animals that lost the MEC-12-expressing array from the PLM. No array, non-transgenic animals. Scale bars  = 5 µm.