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Internalization is temperature-dependent and uses endocytic routes. Confocal images of live CHO-K1 cells after incubation in the presence of the general endocytosis marker FM4-64 (red channel). Cells were incubated at (A) 4°C with 10 μM peptides or (B) 37°C using 5 μM (green channel), concentration of FM4-64 was 5 μg/ml in both cases. Laser and photomultiplier settings were kept constant for each temperature; intensities within the respective panels are therefore comparable.
