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. 2014 Nov 7;13:149. doi: 10.1186/s12934-014-0149-5

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© Diederichs et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of oxygen transfer rates of all E. coli variants in selected auto-induction media. Oxygen transfer rate during cultivation of E. coli BL21 (DE3) variants expressing different recombinant target proteins (3-hydroxybutyryl-CoA dehydrogenase (HBD), glucose 1-dehydrogenase (GDH), HBD-GDH, and alcohol dehydrogenase A (ADH-A)) in Overnight Express auto-induction medium ((A) OnEx_1 and (B) OnEx_2) and (C) auto-inducing TB+lactose medium (Roth_4). The respiration behavior is categorized into two patterns, as described in the caption of Figure 4. Conditions: 250-mL flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm, and 37°C.