Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 15;25(23):3726–3739. doi: 10.1091/mbc.E14-06-1109

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Zhen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — The association of Cbx2 fusion protein with mitotic chromosomes in PRC1 and PRC2 gene-knockout ES cells. (A) Confocal fluorescence images of YFP-Cbx2 fusion protein expressed in PGK12.1 ES cells (WT-ES). The different version of YFP-Cbx2 of Figure 1A is presented here in order to compare with B and C. Scale bar, 5 μm. (B) Confocal fluorescence images of YFP-Cbx2 fusion protein expressed in Eed^−/− ES cells in various phases of mitosis. Prometaphase (top), metaphase (middle), and anaphase/telophase (bottom). Scale bar, 5 μm. (C) Confocal fluorescence images of YFP-Cbx2 fusion protein expressed in Ring1a^−/−/Ring1b^fl/fl and Bmi1^−/−/Mel18^−/− ES cell lines in various phases of mitosis. Prometaphase (top), metaphase (middle), and anaphase/telophase (bottom). The Ring1a^−/−/Ring1b^fl/fl was treated with OHT to induce depletion of Ring1b for 3 d before imaging. The protein level of Ring1b in Ring1a^−/−/Ring1b^fl/fl cells with or without OHT is presented in Supplemental Figure S5. Scale bar, 5 μm. (D) Quantitative analysis of mitotic binding fractions of YFP-Cbx2 fusion protein at mitotic chromosomes. The data represent at least 10 cells analyzed. Error bars, SD of the mean.