Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 15;25(23):3798–3812. doi: 10.1091/mbc.E14-03-0818

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Prakasam et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — A₁AR-stimulated exocytosis is dependent on protein synthesis and secretion. (A) Cryosection of rat bladder epithelium labeled with an A₁AR-specific antibody (green), rhodamine–phalloidin to label the actin cytoskeleton (red), and TOPRO-3 (blue) to label the nucleus. Right, merge. An umbrella cell is marked with an asterisk and the white arrows mark the apicolateral borders of the cell. Scale bar, 12 μm. (B) Rabbit uroepithelium was mounted in Ussing chambers and after equilibration left untreated (no drug), or 1 μM adenosine or 500 nM CCPA was added to the mucosal hemichamber. Percentage changes in C_T were monitored. (C) Rabbit tissue was left untreated, pretreated with 5 μg/ml brefeldin A (BFA) for 30 min, or pretreated with 100 ng/ml cyclohexamide for 60 min. CCPA (500 nM) was then added to the mucosal hemichamber and C_T recorded. Data for CCPA alone are reproduced from B. (B, C) Mean changes in C_T ± SEM (n ≥ 4). Significant differences (p < 0.05), relative to no drug in A or CCPA in B, are indicated with an asterisk.