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. 2014 Nov 15;25(23):3813–3822. doi: 10.1091/mbc.E14-04-0907

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© 2014 Luo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Ectopic targeting of the exocyst to mitochondria led to secretion defects. (A) Growth of cells expressing Tom20-mCherry–tagged exocyst subunits. The same amount of yeast cells was serially diluted at 1:10 ratio and then spotted on SC plates at 25°C. Except for Tom20-mCherry-Exo70, yeast cells expressing Tom20-mCherry–tagged exocyst subunits grew more slowly than control cells expressing Tom20-mCherry. (B) The growth defect of Tom20-mCherry-Sec3 cells can be suppressed by high-copy 2μ plasmids expressing either SEC1 or SEC4 gene. (C) Bgl2p secretion in cells expressing Tom20-mCherry–tagged exocyst subunits. Except for Tom20-mCherry-Exo70, yeast cells expressing Tom20-mCherry–tagged exocyst subunits showed accumulation of Bgl2p in cells (Internal) detected by Western blotting. Molecular weights (in kilodaltons) are indicated to the left. Alcohol dehydrogenase (Adh1p) was used as a loading control. (D) Except for Tom20-mCherry-Exo70, yeast cells expressing Tom20-mCherry–tagged exocyst subunits were defective in invertase secretion at 25°C. The percentage of external (secreted) relative to the total invertase is presented. Error bars represent SD (n = 3). *p < 0.01 as compared with cells expressing Tom20-mCherry.