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. 2014 Nov 15;25(23):3813–3822. doi: 10.1091/mbc.E14-04-0907

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© 2014 Luo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Ectopic expression of Sec3p is required for exocyst targeting and assembly at mitochondria. (A) Sec6-GFP and Sec8-GFP can be recruited to mitochondria in cells expressing Tom20-mCherry tagged Sec3C (amino acids [aa] 321–1336) but not Sec3N (aa 1–320). (B) Cells expressing Tom20-mCherry-Sec3p or -Sec3C grew more slowly than cells expressing Tom20-mCherry-Sec3N on a synthetic complete plate at 25°C. (C) Cells expressing Tom20-mCherry-Sec3 or -Sec3C showed accumulation of Bgl2p inside the cells as detected by Western blotting. Adh1p was used as a loading control. Molecular weights (in kilodaltons) are indicated to the left.