FIGURE 5:

Sec2p and Sec4p control exocyst assembly at the mitochondria in cells expressing Tom20-mCherry-Sec3p. Localization of Sec8-GFP was examined in wild-type, sec1-1, sec2-41, sec4-8, and sec9-4 cells expressing Tom20-mCherry or Tom20-mCherry-Sec3p. In mutant cells expressing Tom20-mCherry, Sec8-GFP was located to the bud at the permissive temperature (25°C) and became diffused in the cytoplasm at the restrictive temperature (37°C). In cells expressing Tom20-mCherry-Sec3p, Sec8-GFP was recruited to mitochondria at 25°C. At 37°C, Sec8-GFP remained associated with mitochondria in wild-type, sec1-1, and sec9-4 cells but diffused in sec2-41 and sec4-8 cells.