Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 15;25(23):3823–3833. doi: 10.1091/mbc.E14-06-1140

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Omnus and Ljungdahl. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — Turnover of nuclear-localized Stp1 is Asi dependent. (A) Immunoblot analysis of extracts from MBY89 (ssy5Δ; WT), MBY106 (ssy5Δ asi1Δ), MBY108 (ssy5Δ asi2Δ), and MBY110 (ssy5Δ asi3Δ) carrying pAB27 (RI_15-35) or pDO74 (RI_17-33) as indicated. (B) Immunoblot analysis of cell extracts from MBY93 (ssy5Δ stp1Δ stp2Δ), MBY102 (ssy5Δ stp1Δ stp2Δ asi1Δ), CAY86 (grr1Δ), and CAY109 (grr1Δ asi1Δ) carrying pCA047 (STP1), pAB27 (RI_15-35), pCA120 (STP1-133), or pDO248 (STP1ΔR1) treated with CHX. Cells were pregrown in SD; samples were taken 0 and 10 min after cultures received an aliquot of CHX (final concentration, 100 µg/ml). Levels of Pgk1 serve as internal control for protein loading.