TABLE 2:
Plasmids used in this study.
| Plasmid | Description | Reference/source |
|---|---|---|
| pAB1 | pRS313 carrying HIS3, MET15, and LEU2 | Omnus et al. (2011) |
| pAB27 | pRS316 (URA3) expressing Stp1-RI15-35 | This study |
| pCA047 | pRS316 (URA3) with STP1-3HA | Andréasson and Ljungdahl (2002) |
| pCA072 | pRS316 (URA3) with FLAG-STP1-HA | Andréasson and Ljungdahl (2002) |
| pCA078 | pRS202 (URA3) with FLAG-STP1-HA | Andréasson and Ljungdahl (2002) |
| pCA094 | URA3 derivative of pSOS | Andréasson and Ljungdahl (2002) |
| pCA104 | pCA094 (URA3) expressing hSOS-Stp12-158 | Andréasson and Ljungdahl (2002) |
| pCA120 | pRS316 (URA3) with STP1-133 | Andréasson and Ljungdahl (2002) |
| pDO39 | pRS316 (URA3) expressing Stp11-125-Htb2-GFP-HA | This study |
| pDO73 | pRS316 (URA3) expressing Stp1-RI22-35 | This study |
| pDO74 | pRS316 (URA3) expressing Stp1-RI17-33 | This study |
| pDO141 | pRS316 (URA3) with STP1-134 | This study |
| pDO142 | pRS316 (URA3) with STP1-135 | This study |
| pDO196 | pCA104 (URA3) expressing hSOS-Stp12-158(Δ16-21) | This study |
| pDO203 | pCA104 (URA3) expressing hSOS-Stp12-64 | This study |
| pDO205 | pCA104 (URA3) expressing hSOS-Stp116-33 | This study |
| pDO211 | pEG202 derivative (HIS3) KanR carrying PADH1-lexA-AD | This study |
| pDO221 | pCA104 (URA3) expressing hSOS-Stp116-64 | This study |
| pDO230 | pCA104 (URA3) expressing hSOS-Stp116-37 | This study |
| pDO231 | pCA104 (URA3) expressing hSOS-Stp116-50 | This study |
| pDO237 | pRS316 (URA3) with STP1Δ127-150-3HA | This study |
| pDO243 | pRS316 (URA3) with STP1Δ94-113-3HA | This study |
| pDO244 | pRS316 (URA3) with STP1Δ288-306-3HA | This study |
| pDO246 | pRS316 (URA3) with STP1Δ365-420-3HA | This study |
| pDO247 | pRS316 (URA3) with STP1Δ443-471-3HA | This study |
| pDO248 | pRS316 (URA3) with STP1ΔR1-3HA | This study |
| pDO260 | pEG202 derivative (HIS3) KanR carrying PADH1-STP116-50-lexA-AD | This study |