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. 2014 Jun 25;14:169. doi: 10.1186/1471-2180-14-169

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Copyright © 2014 Rydzewski et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Thin-section EM of Francisella strain W12-1067. (A) Bacteria cultivated in medium T at 37°C possess a rod-shaped, slightly pleomorphic morphology. (B-D) Incubation of J774A.1 cells with bacteria (MOI 10). (B) Overview of host cells with two compartments containing bacteria (96 h post infection). Rectangles mark regions shown at higher magnification in C and D, respectively. (C) Two bacteria in a membrane-bound compartment. (D) Several bacteria in a compartment which shows a clearly discernible membrane at least in some regions.