Table 5.
Genes of the putative T6SSs I and II of Francisella isolate W12-1067
| Name (T6SS I) | Peg Nr. (aa) | kDa | Feature | Closest homolog (% aa identity) |
|---|---|---|---|---|
| OrfD1 |
950 (92) |
9.9 |
DUF2345, VgrG-like, Rhs family |
FTN_0038 (57%) |
| OrfC1 |
951 (138) |
15.7 |
SP, lipid attachment site (IglE-like) |
FTN_0039 (66%) |
| OrfB1 |
952 (1097) |
127.1 |
SP, TM domain (PdpB-like/TssM) |
FTN_0040 (58%) |
| OrfA1 |
953 (735) |
84.6 |
Cl* |
FTN_0041 (63%) |
| Orf48 |
954 (48) |
5.8 |
SP |
--- |
| ImpB1 |
955 (179) |
20.8 |
homologous to IglA/TssB |
FTN_0042 (96%) |
| ImpC1 |
956 (508) |
57.7 |
homologous to IglB/TssC |
FTN_0043 (91%) |
| OrfE1 |
957 (214) |
23.1 |
helical bundle domain (putative TssD) |
FTN_0044 (77%) |
| OrfF1 |
958 (356) |
41.3 |
Cl |
FTN_0045 (51%) |
| OrfG1 |
059 (406) |
47.3 |
Cl, T6SS-associated like protein |
FTN_0048 (72%) |
| OrfH1 |
960 (274) |
31.9 |
C-terminal TM domain |
FTN_0049 (63%) |
| OrfI1 |
961 (501) |
58.6 |
Cl |
FTN_0050 (72%) |
| OrfJ1 |
962 (203) |
23.8 |
DUF2077 (putative DotU/TssL) |
FTN_0051 (63%) |
| OrfK1 |
963 (126) |
13.1 |
DUF4280 |
FTN_0054 (82% |
| Orf374 |
964 (374) |
43.3 |
Cl |
Hyp. protein (33%) Flavobacterium |
| IglD3 |
1538 (398) |
46.1 |
DUF876 (TssK) |
IglD (49%) F-TX077308 |
|
Name (T6SS II) |
Peg Nr. |
kDa |
Feature |
Closest homolog (% aa identity) |
| ImpB2 |
1376 (177) |
19.9 |
Cl, homologous to IglA/TssB, DUF770 |
FTN_0042 (42%) |
| ImpC2 |
1375 (493) |
55.5 |
Cl, homologous to IglB/TssC, COG3517 |
FTN_0043 (47%) |
| Orf204 |
1374 (204) |
22.4 |
Cl, |
--- |
| OrfG2 |
1373 (405) |
47.1 |
Cl, IglD-like |
F7308_1914 (28%) |
| Orf368 |
1372 (368) |
43.4 |
Cl |
--- |
| OrfL |
1371 (629) |
74.6 |
Hypothetical protein |
F7308_1916 (33%) |
| Orf96 |
1370 (96) |
11.1 |
2 TM domains |
Hyp. protein (49%) Prevotella histicola |
| OrfB2 |
1369 (928) [1020] |
106.3 |
Cytoplasmic membrane, SP |
FTN_0040 (20%) |
| Orf457 |
1368 (457) |
53.4 |
Hypothetical protein |
--- |
| Orf254 |
1367 (254) |
30.3 |
Cl |
--- |
| OrfC2 |
1366 (146) |
17 |
SP |
FTN_0039 (24%) |
| OrfJ2 |
1365 (195) |
23.1 |
DUF2077 (putative DotU/TssL) |
DotU, (29%) Desulfonatronospira |
| OrfD2 |
1364 (111) |
12.4 |
Cl (DUF2345, vgrG) |
--- |
| ORF705 | 1363 (705) | 77.7 | Cl | --- |
*determined by using “psortb” (http://www.psort.org/psortb); Cl, cytoplasmic localization; TM, transmembrane domain; SP, signal peptide; (aa), number of amino acids.