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Efficiency of protoplast isolation from A95 cells using different cell-wall lysing enzymes
| Enzymes used for protoplast isolation | Starting number of A95 cells (cfu.mL −1 ) | Number of protoplasts isolated.mL −1 |
|---|---|---|
| Lysing enzymes from Trichoderma harzianum |
2 × 107 |
2.0 × 106 |
| β-Glucanase, Vinoflow, Yatalase |
2 × 107 |
1.9 × 106 |
| Driselase, β-Glucanase, Lyticase | 2 × 107 | 1.1 × 106 |
