Table 2.
Mean [±S.D] cellular sterol composition (%) of turbo yeast and MYA-796
| |
Sterol composition (%) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
Zymosterol |
Ergosterol |
Lanosterol |
a
Intermediates |
Cholesterol |
|||||
| Turbo | MYA-769 | Turbo | MYA-769 | Turbo | MYA-769 | Turbo | MYA-769 | Turbo | MYA-769 | |
|
YPD |
17.92 [0.38] |
17.79 [0.49] |
61.71 [3.29] |
61.91 [1.39] |
5.93 [1.65] |
8.06 [0.59] |
14.45 [2.02] |
12.25 [1.49] |
— |
— |
|
GJ + tfosEp |
15.53 [2.80] |
12.31 [0.10] |
67.31 [0.49] |
61.86 [1.10] |
5.90 [0.09] |
9.31 [0.76] |
11.26 [3.20] |
16.52 [0.23] |
— |
— |
| glc YM +chol | 5.7 [0.78] | 1.6 [0.41] | 26.9 [1.27] | 12.2 [2.12] | 35.7 [0.74] | 38.2 [3.64] | 27.10 [1.79] | 27.30 [2.05] | 4.6 [0.48]b | 20.7 [0.96]b |
The most abundant sterol in each experiment is emboldened. a = sum of all minor sterol intermediates (each comprising < 5% of total cellular sterol fraction); b = exogenously supplied cholesterol; Strikethrough = not detected.