Fig. 1.

Schematic diagram of the multimodal nonlinear optical imaging (TPEF/THG) platform for label-free imaging of live cells. FM, flip mirror; L, lens; M, mirrors; OPO, optical parametric oscillator; MO, microscope objective; PCF, photonic crystal fiber; F1, filter set 1 (600 ± 20nm bandpass filter); F2, filter set 2 (730 nm excitation: 650 nm shortpass, 460 ± 30nm bandpass filter; 860 nm excitation: 750 nm shortpass, 540 ± 20nm bandpass filter; 600 nm excitation: 532 nm shortpass, 355 ± 20nm bandpass filter); F3, filter set 3 (650 nm shortpass filter, 390 ± 20nm bandpass filter); DM, dichroic mirror; PMT, photomultiplier tube.