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. 2014 Nov 13;10(11):e1004800. doi: 10.1371/journal.pgen.1004800

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© 2014 Erhardt et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Secretion of the Spi1 vT3SS substrate InvJ or a 3×HA tagged InvJ variant. (A) Secreted InvJ protein in the wildtype, ΔinvJ, ΔatpA, the catalytically-inactive Spi1 ATPase invC^K165E, invC^K165E ΔatpA, invC^K165E ΔflgM, and invC^K165E ΔflgM ΔfliHIJ ΔssaN ΔatpA mutant strains. Detection of DnaK protein was included as a cell lysis control. (B) Levels of secreted InvJ::3×HA protein in the wildtype, ΔinvJ::3×HA, ΔinvJ::3×HA ΔatpA, ΔinvJ::3×HA invC^K165E, and ΔinvJ::3×HA invC^K165E ΔatpA mutant strains. 288 ng BSA was added to each supernatant fractions (except for lane 2) and served as a precipitation control. DnaK protein served as a cell lysis control.