Table 1. List of Salmonella enterica servovar Typhimurium LT2 strains used in this study.

Strain number	Relevant genotype	Reference
TH437	wildtype	J. Roth
TH6232	Δhin-5717::FRT	[47]
TH8208	Δhin-5717::FRT fliA*5225(H14D)	Lab collection
TH8419	ΔinvJ::FKF	Lab collection
TH9949	flgE6569::bla ΔflgBC655	[35]
TH11801	ΔatpA::tetRA	[15]
TH12465	flgE6569::bla ΔflgBC655 ΔfliF7387	This study
TH12472	flgE6569::bla ΔflgBC655 ΔfliH7394	This study
TH12473	flgE6569::bla ΔflgBC655 ΔfliI7395	This study
TH12474	flgE6569::bla ΔflgBC655 ΔfliJ7396	This study
TH12475	flgE6569::bla ΔflgBC655 ΔfliHI7397	This study
TH12476	flgE6569::bla ΔflgBC655 ΔfliHIJ7398	This study
TH12477	flgE6569::bla ΔflgBC655 ΔfliIJ7399	This study
TH13867	Δhin-5717::FRT ΔfliI7364	This study
TH13868	Δhin-5717::FRT ΔfliHI7366	This study
TH13869	Δhin-5717::FRT ΔfliHIJ7367	This study
TH14002	Δhin-5717::FRT clpX1::Tn10dCm ΔfliHI7366	This study
TH14009	Δhin-5717::FRT ΔflgM5628::FRT ΔfliI7364	This study
TH14010	Δhin-5717::FRT ΔflgM5628::FRT ΔfliHI7366	This study
TH14011	Δhin-5717::FRT ΔflgM5628::FRT ΔfliHIJ7367	This study
TH14017	Δhin-5717::FRT fliA*5225 (H14D)	This study
TH14018	Δhin-5717::FRT fliA*5225 (H14D) ΔfliHI7366	This study
TH14019	Δhin-5717::FRT fliA*5225 (H14D) ΔfliHIJ7367	This study
TH14129	Δhin-5717::FRT ΔfliHI7366 ΔclpX	This study
TH14130	Δhin-5717::FRT clpX1::Tn10dCm ΔfliHI7366 atp (Mot+)	This study
TH14184	ΔfliHI7366 Δhin-5717::FRT ΔclpX ΔatpA::tetRA	This study
TH14260	ΔflgM5628::FRT ΔfliHI7366 Δhin-5717::FRT ΔatpA::tetRA	This study
TH14261	fliA*5225 (H14D) ΔfliHI7366 Δhin-5717::FRT ΔatpA::tetRA	This study
TH14292	ΔfliHI7366 Δhin-5717::FRT ΔatpA::tetRA	This study
TH14826	Δhin-5717::FCF ΔflgM5628::FRT	This study
TH15426	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT)	This study
TH15427	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliF7387	This study
TH15430	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliI7395	This study
TH15431	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliHIJ7398	This study
TH15925	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliJ7396	This study
TH16031	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliH7394	This study
TH16032	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliHI7397	This study
TH20154	invC314 (K165E)	This study
TH20637	invC314 ΔatpA::tetRA	This study
TH20708	invC314 ΔflgM5628::FRT ΔfliHIJ7367 ΔssaN110	This study
TH20709	invC314 ΔflgM5628::FRT ΔssaN110 ΔfliHIJ7367 ΔatpA::tetRA	This study
TH20714	invJ320::3×HA (SAGASA linker on both sides of 3×HA; between aa140 and 141)	This study
TH20750	invJ320::3×HA ΔatpA::tetRA	This study
TH20830	invC314 invJ321::3×HA (SAGASA linker on both sides of 3×HA; between aa140 and 141)	This study
TH20831	invC314 invJ322::3×HA (SAGASA linker on both sides of 3×HA; between aa140 and 141) ΔflgM5628::FRT ΔfliHIJ7367 ΔssaN110	This study
TH20832	invC314 invJ321::3×HA ΔatpA::tetRA	This study
TH20833	invC314 invJ322::3×HA ΔflgM5628::FRT ΔfliHIJ7367 ΔssaN110 ΔatpA::tetRA	This study
EM404	Δhin-5717::FRT ΔflgM5628::FRT	This study
EM405	Δhin-5717::FRT ΔatpA::tetRA	This study
EM406	Δhin-5717::FRT ΔfliI7364 ΔatpA::tetRA	This study
EM407	Δhin-5717::FRT ΔfliHIJ7367 ΔatpA::tetRA	This study
EM408	Δhin-5717::FRT ΔflgM5628::FRT ΔfliI7364 ΔatpA::tetRA	This study
EM409	Δhin-5717::FRT ΔflgM5628::FRT ΔfliHIJ7367 ΔatpA::tetRA	This study
EM415	Δhin-5717::FRT ΔflgM5628::FRT ΔatpA::tetRA	This study
EM417	Δhin-5717::FRT ΔclpX	This study
EM1236	flgE6569::bla ΔflgBC655 PflhD7793 (P1+P4 -10 TATAAT) ΔfliIJ7399	This study
EM1959	Δhin-5717::FRT flhA22345 (D208A) ΔatpA::tetRA	This study
EM1960	Δhin-5717::FRT flhA22346 (D208E) ΔatpA::tetRA	This study
EM1961	Δhin-5717::FRT flhA22347 (D208K) ΔatpA::tetRA	This study
EM2037	Δhin-5717::FRT flhA22345 (D208A) ΔflgM5628::FKF	This study
EM2038	Δhin-5717::FRT flhA22346 (D208E) ΔflgM5628::FKF	This study
EM2039	Δhin-5717::FRT flhA22347 (D208K) ΔflgM5628::FKF	This study