Table 1.
Bacterial strains, plasmids and oligonucleotides used in this study
| Description | Reference or source | |
|---|---|---|
|
Strains |
|
|
|
Ralstonia eutropha H16 |
Wild type |
DSM 428 |
|
Escherichia coli |
|
|
| BL21 (DE3) |
F−ompT hsdSB (rB−, mB−) gal dcm (DE3) |
Novagen |
|
Plasmids |
|
|
| pCOLADuet-1::3HAD |
pCOLADuet-1 with fadB’ as BamHI/HindIII fragment |
Volodina et al. [2014] |
| pET-19b::pct |
pET-19b with pct as NdeI/XhoI fragment |
Lindenkamp et al. [2013] |
| pET-23a |
E. coli expression vector (Apr, T7 promoter) |
Novagen |
| pET-23a::bktB |
pET-23a with bktB as NdeI/HindIII fragment |
This study |
|
Oligonucleotides |
|
|
| Forward primer BktB NdeI* |
AAACATATGACGCGTGAAGTGGTAGTGGTAAGC |
This study |
| Reverse primer BktB HindIII* | AAAAAGCTTGATACGCTCGAAGATGGCGGC | This study |
*Restriction sites are highlighted in italics.