Table 2.

Enzyme characteristic of FadB’ in comparison with other enzymes

	Cofactor		Trifunctional activity	Specificity to ( S )- or ( R )-3-hydroxyacyl-CoA	Reference
	NAD +	NADP +
FadB’Re	100*	5*	+	S**	this work
PhaB1Re	20	100	-	R	Haywood et al. [1988b]
Unidentified acetoacetyl-CoA reductase (R. eutropha H16)	100	2	n.a.	S/R	Haywood et al. [1988b]
FadBEc	+	n.a.	+	S	Binstock and Schulz [1981]

*-The activity of FadB’ was measured at 340 nm and 30°C with acetoacetyl-CoA in direction of 3-hydroxybutyryl-CoA formation as described in “Materials and Methods”.

**-The stereospecificity of FadB’ was measured at 303 nm and 30°C with (S)- and (R)-3hydroxybutyryl-CoA as described in “Materials and Methods”.