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. 2014 Nov 13;8(11):e3308. doi: 10.1371/journal.pntd.0003308

Figure 1. TLR9-dependent activation of BMDCs specific to Trypanosomatidae DNA.

Figure 1

C57BL/6 or TLR9-/- BMDCs were stimulated in vitro either with Trypanosomatidae or vertebrate DNA for 6 hours (A–C) IL-6 and TNFα production was measured by ELISA in the supernatants of stimulated BMDCs for 6 h (A) with Trypanosomatidae DNA alone or complexed with DOTAP, (B) with different concentrations of L. major or vertebrate (mouse or pig) DNA alone or complexed with DOTAP, (C) with CpG 1826 (0,25 µg/ml) and LPS (100 ng/ml) as controls, (D) with L. major or mouse DNA sonicated in 200 base pair (bp) fragments. The data represent the mean and SEM of three independent experiments. Significant differences were found between C57BL/6 and TLR9-/- in A, B, C (*, p<0.05) and between L. major and vertebrate DNA (mouse or pig) in C and D (*, p<0.05; **, p<0.01; ***, p<0.001).