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. 2014 Nov 13;8(11):e3308. doi: 10.1371/journal.pntd.0003308

Figure 2. Stimulation of human plasmacytoid dendritic cells by L. major and vertebrate DNA.

Figure 2

Human plasmacytoid cell line (Gen2.2) was stimulated by L. major, mouse and human DNA alone (left) or complexed with DOTAP (right). In the indicated lanes, cells were treated with chloroquine (20 µM) before L. major stimulation. Expression of the indicated cytokines was determined by real time RT-PCR. The mRNA expression levels were normalized to the expression of the HPRT gene and calculated as the n-fold difference with the expression in unstimulated cells. The results represent the mean and SEM of three independent experiments (*p<0,05, **p<0,01).