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. 2014 Nov 13;8(11):e3308. doi: 10.1371/journal.pntd.0003308

Figure 5. Competition with vertebrate DNA prevents the immunostimulatory activity of L. major DNA.

Figure 5

C57BL/6 BMDCs were stimulated in vitro for 6 h with 10 µg of L. major DNA (A–B) or T. cruzi DNA (C), alone or with 10 µg of vertebrate DNA. Cytokines production was measured by ELISA in the supernatants of cultures. (A and C) The data represent the mean and SEM of three independent experiments. Significant differences are indicated (*, p<0.05; **, p<0.01; ***, p<0.001). (B) The percentage of inhibition of BMDCs activation obtained by adding increasing amount of vertebrate DNA (mouse, pig or sheep) to L. major DNA. Percentage (%) of inhibition  =  [100-{cytokines production by L. major with vertebrate DNA/cytokines production by L. major DNA alone}]×100. The results represent the mean and SEM of three independent experiments.