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. 2014 Nov 13;8(11):e3309. doi: 10.1371/journal.pntd.0003309

Figure 8. Evaluation of role of LicNTPDases in the infection and adhesion of L. infantum chagasi to macrophages.

Figure 8

(A) Adhesion of Leishmania promastigotes to macrophages. (B) Infection of macrophages by Leishmania promastigotes. (C) Treatment did not affect the amount of parasites in macrophages. In all assays (A, B and C): Macrophages treated with polyclonal antiserum anti-rLicNTPDase-2 prior to the infection or with the purified rLicNTPDase-2 before the contact with parasites are compared with macrophages treated with the parasites in the absence of intervention. Control  =  adhesion and infection assay without any intervention. Control with enzyme buffer  =  adhesion and infection assay in the presence of the buffer used to suspend rLicNTPDase-2. BSA was used as a non-related protein. The data reflect the mean + SE from three analyzed slides from each of three independent assays. The asterisks indicate significant differences (p<0.05) between the control and other samples.