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. 2014 Nov 13;9(11):e111850. doi: 10.1371/journal.pone.0111850

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© 2014 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Flow cytometry analysis of cultured MSCs. Both MSCs from young and old donor were positive for CD90, CD29 and negative for haematopoietic markers CD45FITC and CD34PE. B. Morphology and multiplex differentiation potential of cultured MSCs. The presence of adipocytes was verified by staining for triglycerides (red) with Oil Red O and the presence of active osteoblasts were verified by staining for calcium deposition (red) with Alizarin Red S. Representative images were shown. Scale bar: 100 µm.