Figure 4. Collagen accumulation in wound areas of HYAL and vehicle-control treated rats at day 2, 7, 14 and 21 after wounding.

(A) Representative photomicrograph of wounds tissue sections stained with picrosirius red staining (200x), note the collagen intensity and disposition of fibers (red). (B) Collagen content measured by digital densitometry is shown as a result of collagen content in each specimen in percentage. (C) Determination of wound hydroxyproline content as an indicator of collagen levels. µg of hydroxyproline/mg of dry wound specimen content was measured at day zero, 2, 7, 14 and 21 after injury. Data represent means ± SEM (n = 8 wounds/group), *P<0.05, **P<0.01 compared to control group by one-way-ANOVA.