Figure 3. Reduction in IM injection volume increases transgene expression.

10¹⁰ GC AAV8 expressing ffLuc from the CMV or TBG promoter was delivered IM as either two 25 µl injections (one into each leg), one 10 µl injection or one 2 µl injection to C57BL/6 mice. IV injections were performed as a 100 µl injection via the tail vein. ffLuc tissue assays were performed on tissue harvested at day 28 and normalized to the total protein concentration of liver (A) and muscle (B). RAG KO mice were administered with 10¹⁰ GC AAV8.CMV.201Ig IA (C) or AAV8.TBG.201Ig IA (D) by IV or IM injections, performed as described previously. Data for the one 10 µl injection groups were previously presented in Figure 1. Expression of 201Ig IA in serum was measured by ELISA. Values are expressed as mean ± SEM (n = 4/group). *p<0.05, **p<0.01, ***p<0.001.