Table 1.
Primers used for cloning and real-time RT-PCR
| Gene name | Sequence (5′ to 3′) S, Sense; AS, Antisense | Accession # | Size (bp) | Application |
|---|---|---|---|---|
| Actg2 | S: CGCCCTAGACATCAGGGT AS: TTCTGGTGCTACTCGAAGC |
NM_009610 | 189 | Real-time RT-PCR |
| Acvr2a | S: CGGCATTGTTTTGCTACCTG AS: TGTGTGACTTCCATCTCCGG |
XM_005068951 | 200 | Real-Time RT-PCR |
| Atp1b1 | S: ATGGGTTGTGTTGTGCTCC AS: ACATGATGCCTCCAGAGA |
XM_005071373 | 179 | Real-time RT-PCR |
| Cd24a | S: ACCCACGCAGATTTACTGCA AS: CGTTTCCTGGCCTGAGTCTC |
NM_009846 | 191 | Real-time RT-PCR |
| E2f8 | S: GAGAAATCCCAGCCGAGTC AS: CATAAATCCGCCGACGTT |
NM_001013368 | 157 | Real-time RT-PCR |
| E2f8 | S: CAGTCAAGTGAACCCAGGAA AS: CGGGTTGTAAGTAGATGGCA |
XM_005083212 | 234 | Cloning |
| Fst | S: GCCTGTGGGATTTCAAGGTT AS: CAGCTTCCTTCATGGCACAC |
XM_005082770 | 135 | Real-time RT-PCR |
| Fst | S: TCTGCCAGTTCATGGAGGAC AS: CACATTCGTTGCGGTAGGTT |
XM_005082770 | 378 | Cloning |
| Gadd45a | S: CGTGCTAGTGACGAACCCAC AS: ATTGAGATGCCATCACCGTT |
XM_005076070 | 139 | Real-time RT-PCR |
| Hnrnpd | S: ATCCTATCACAGGGCGATCA AS: GGCCCGTTTAGGATCAATGA |
XM_005068122 | 125 | Real-time RT-PCR |
| Hoxb6 | S: AACAGTTCCTCTTTTGGGCC AS: TCCTTTTTCCACTTCATGCG |
XM_005075884 | 200 | Real-time RT-PCR |
| Hprt | S: CTTGCTGGTGAAAAGGACCTC TCGAAG AS: TGAAGTACTCATTATAGTCA AGGGCAT |
NM_013556 | 115 | Real-time RT-PCR |
| Itm2b | S: AGCTGCTCGCTACCAGACAA AS: GATGGACGTGTTCAGAGGA |
XM_005070971 | 197 | Real-time RT-PCR |
| Klf9 | S: CAGTCTGGAGAGTCCCGATG AS: CCAGAGTGGAGGAGGGAGA |
XM_005063225 | 139 | Real-time RT-PCR |
| Lmna | S: GCTGCACTGAGCACTGCTCT AS: GGGTCTGCAGCCTGTTCTC |
XM_005080125 | 163 | Real-time RT-PCR |
| Nppc | S: AGCGGTCTGGGATGTTAGTG AS: CCTCCCCTCCCCAAATAATA |
NM_010933 | 202 | Real-time RT-PCR & Cloning |
| Prdx4 | S: CGGATCACTCCCTGCATCTA AS: TGAGCTCCTTGAATTCTCCG |
NM_016764 | 96 | Real-time RT-PCR |
| Psmb3 | S: GTGACCACGGACTTCCAGAA AS: TACAGAAGGTTGGCCACCAT |
XM_005139336 | 185 | Real-Time RT-PCR |
| Ran | S: CTTGGAGTTTGTTGCCATGC AS: TCCAGCTTCGCTTTCTCACA |
XM_005079847 | 148 | Real-Time RT-PCR |
| Ran | S: TTTGTTGCCATGCCTGCTC AS: GCCCATTCATCTCCTTCAGC |
XM_005079847 | 265 | Cloning |
| Ube2c | S: CCCCAGTGGCTACCCTTACA AS: TGCGGACCACTTATCCTTGA |
XR_219744 | 118 | Real-time RT_PCR |
| Ube2c | S: CCTGAATCAGACAACCTGTTC AS: GCGGACCACTTATCCTTGAG |
XR_219744 | 212 | Cloning |
| Ypel3 | S: ACAGGTCTTCATGCTGTCGC AS: CCAGCCGTTGTCTTTGATCA |
XM_005064410 | 150 | Real-Time RT-PCR |