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. 2014 Nov 13;9(11):e112571. doi: 10.1371/journal.pone.0112571

Figure 1. NanoLuc (Nluc) luminescence in Plasmodium falciparum.

Figure 1

(A) Diagrams of firefly and Nluc reporter vectors. (B) pPf86 and pPfNluc were transfected in trophozoite stage parasites and luciferase activity in relative light units (RLU) determined 4 days later. Note that the RLU of each luciferase was measured in its own optimal substrate ie, Nluc with Nano-Glo and Firefly with D-luciferin. Mock-transfected parasites were used as negative control and to determine background luminescence, which was then subtracted from firefly and Nluc activities. The result represents the mean of 3 independent transfections ± standard deviation.