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. 2014 Nov 13;9(11):e112571. doi: 10.1371/journal.pone.0112571

Figure 4. Quantification of Nluc in cellular compartments of infected RBCs.

Figure 4

(A) Schematic of the iRBC’s compartments where RBC represents the exported fraction that is released after Equinatoxin treatment. The PV compartment was then released following treatment with 0.01% saponin and finally, the Parasite fraction was lysed by Nano-Glo Luciferase Assay Reagent. (B) Trophozoite stage parasites transfected with either the original Nluc, secreted SP-Nluc or the exported PEXEL-Nluc fusions were fractionated as shown in (A) and luciferase activity measured. Nluc activities as a percentage of the total for each parasite line are shown and represent the mean of 3 experiments +/−SEM. Similar to (B), (C) Schizonts and (D) Ring stage parasites were also fractionated. Statistical significance (* p<0.05) was determined by 2 way ANOVA test comparing the percentage of reporter activity of each sub-cellular fraction among the 3 cell lines.