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. 2014 Nov 13;9(11):e112571. doi: 10.1371/journal.pone.0112571

Figure 6. Quantification of NPP activity and inhibition in PEXEL-NLuc parasites.

Figure 6

Trophozoite stage parasites (3% parasitemia) expressing exported PEXEL-NLuc were incubated with different concentrations of the new permeability pathway (NPP) inhibitor furosemide (µM), and then treated with sorbitol lysis buffer at time zero to induce the release of PEXEL-Nluc which was measured over time. A 1% Triton-X100 in PBS (TX100) treatment was included to simulate rapid lysis and a PBS control for background lysis. Data points represent the mean +/− SD of three replicates.