Table 1. Hematopoietic stem progenitor cells (HSPC) expansion and reticulocyte differentiation for three different sources HSPCs (6 independent experiments were carried out for each HSPC source).

HSPC sources	Mean proportion (%) of reticulocytes at D14 (± SD) (n = 6)	Cell number mean fold increase (± SD) (n = 3)
		After 5 days of expansion	After 7 days of differentiation
UCB	18.3±1.3	11.5±2.3	33.5±2.4
BM	20.5±1.5	3.1±0.3	8.6±0.5
PBMC	32±6	1.3±0.2	3.4±0.2

Results show the mean proportions of reticulocytes observed at the peak of enucleation after 14 days of culture, as well as the increase in total cell number after 5 days of expansion and 7 days of differentiation (3 independent experiments). After 5 days of expansion, the number of cells was counted and divided by the initial number of plated MACS/CD34⁺ cells, while after 7 days of HSPC differentiation, the cell count was compared to the number of cells not previously expanded, results are expressed in mean fold increase (± SD).