Figure 7. No obvious changes in epithelial goblet cell density and mucin expression in middle ears of mutant mice.
(A–B) Mucicarmine staining shows rose-stained goblet cells (arrows) in the wild-type (A) and mutants (B). There was no significant difference in goblet cell density between the wild-type and the mutants. (C) Analysis of expression level of Mucin5ac and Mucin5b by real-time PCR. There was no statistical significance in expression between the wild-type and the mutant mice (n = 8, each genotype, P>0.05). WT, wild-type; M: mutant. Muc5ac, Mucin5ac; Muc5b, Mucin5b.