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. 2014 Nov 13;9(11):e113069. doi: 10.1371/journal.pone.0113069

Figure 3. Agarose gel electrophoresis of the RNA display system vector construction.

Figure 3

Panel A) Lanes 1–3: positive recombinant pUCHisJ plasmid clone (cl) restricted with AflII and NsiI; Lanes 5–7: positive pUCtHisF and Lane 8: negative clone. Panel B) Lanes 2–6: positive recombinants pBRT7QβHisJ restricted with AflII and NsiI; Lane 7: negative clone. Panel C) Lanes 2–7: positive recombinants pBRT7QβtHisF restricted with AflII and NsiI. Panel D): Lane 1: pQβ8 negative control; Lanes 2 and 3: positive recombinants pQβ8ΔA1; Lanes 4 and 5: positive recombinants pBRT7Qβ-FMDV; Lanes 6 and 7: positive recombinants pBRT7QβΔA1 all restricted with NheI. Lanes “ladder” were loaded with the 100 bp or 1 kb DNA ladder.