Table 1. Effects of diapause and other conditions on dilp2-3 mutant flies.
| Week | Hemolymph | Body | |||||||
| Protein (µg/µL) | Glucose (mM) | Trehalose (mM) | Glycogen (µg/mgwm) | Glucose (µg/mgwm) | Trehalose (µg/mgwm) | TAG (µg/mgwm) | Protein (µg/mgwm) | ||
| Control (C) | C0 | 6.96±0.23 | 13.9±0.6 | 0.12±0.02 | 3.95±0.26 | 5.97±0.34 | 1.63±0.37 | 0.45±0.06 | 43.5±0.8 |
| C1 | 3.94±0.10 | 9.97±0.21 | 0.11±0.01 | 15.2±0.4 | 7.48±0.07 | 1.02±0.07 | 0.37±0.02 | 45.8±0.5 | |
| vs C0 | *** | ## | N.S. | *** | N.S. | N.S. | *** | N.S. | |
| Diapause (D) | D1 | 6.81±0.19*** | 16.8±0.6# | 0.41±0.11* | 10.3±0.3*** | 10.7±0.6*** | 3.64±0.48 | 1.21±0.08*** | 46.4±0.6 |
| D2 | 5.46±0.12* | 16.3±0.8# | 0.47±0.08** | 18.2±1.1 | 12.2±0.3*** | 5.59±1.55 | 1.19±0.13** | 38.9±1.3*** | |
| D3 | 5.59±0.39* | 19.3±2.7## | 0.48±0.08** | 18.1±0.9 | 12.8±0.3*** | 7.47±1.16*** | 1.17±0.19** | 37.9±1.1*** | |
| D6 | 5.49±0.45* | 23.5±1.9# | 0.42±0.03** | 14.1±0.7 | 12.8±0.3*** | 5.75±1.22* | 0.81±0.09** | 36.4±0.5*** | |
| D9 | 7.77±0.05*** | 31.5±0.9# | 0.46±0.09* | 11.8±1.1* | 12.1±0.6*** | 2.08±0.29 | 0.86±0.11** | 35.2±0.9*** | |
| D12 | 7.13±0.10*** | 40.8±1.4# | 0.43±0.09* | 12.2±0.3 | 13.5±0.2*** | 2.55±0.73 | 0.87±0.16* | 34.2±0.5*** | |
| Recovery (R) | R1′ | 5.26±0.15 | 20.5±0.4# | 0.26±0.03 | 22.8±1.0*** | 11.0±0.3*** | 2.29±0.56 | 0.49±0.04 | 39.1±0.3** |
| vs D3 | N.S. | N.S. | N.S. | N.S. | N.S. | * | ** | N.S. | |
| R1 | 5.40±0.17** | 24.9±1.3# | 0.30±0.08 | 17.2±0.4 | 10.9±0.2*** | 1.75±0.61 | 0.60±0.07 | 40.1±1.3* | |
| vs D6 | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. | |
| R2 | 6.21±0.25*** | 22.0±1.2# | 0.20±0.03 | 16.3±0.4 | 10.2±0.3** | 1.17±0.04 | 0.79±0.09** | 38.5±0.4*** | |
| vs D6 | N.S. | N.S. | N.S. | N.S. | ** | N.S. | N.S. | N.S. | |
Macronutrient composition in hemolymph and body of flies kept for 1–12 weeks at 11°C and 10L:14D, light/dark (D1–D12) and after recovery for 1 week after 3 weeks of diapause (R1′), or 1 (R1) or 2 weeks (R2) after 6 weeks of diapause. Virgin flies, kept for 1 week at non-diapausing conditions (C1) and recently hatched 3–6 h old flies (C0) were used as a controls. Data are presented as means ± S.E.M, n = 3–5 independent replicates with 10–15 flies in every replicate. Significantly different either from the control (C1) with *p<0.05, ** p<0.01, *** p<0.001 or from the indicated group as assessed by ANOVA, followed by Tukey test or alternatively with # p<0.05, ## p<0.01, ### p<0.001 as assessed by Kruskal-Wallis test followed with Wilcoxon pairwise comparison. N.S. – values are not significantly different. Vs, versus.