Table 2. Effects of diapause and other conditions on dilp5 mutant flies.
| Week | Hemolymph | Body | |||||||
| Protein (µg/µL) | Glucose (mM) | Trehalose (mM) | Glycogen (µg/mgwm) | Glucose (µg/mgwm) | Trehalose (µg/mgwm) | TAG (µg/mgwm) | Protein (µg/mgwm) | ||
| Control | C0 | 5.81±0.15 | 6.97±0.73 | 0.10±0.01 | 4.06±0.28 | 5.31±0.24 | 1.45±0.05 | 0.36±0.01 | 55.6±0.3 |
| C1 | 3.93±0.18 | 9.21±0.23 | 0.10±0.02 | 15.6±0.5 | 7.33±0.17 | 1.21±0.07 | 0.29±0.01 | 43.0±0.8 | |
| vs C0 | ** | N.S. | N.S. | *** | ** | N.S. | ## | *** | |
| Diapause (D) | D1 | 6.09±0.28*** | 10.7±0.7 | 0.16±0.02 | 10.3±0.4*** | 9.08±0.43* | 1.74±0.27 | 0.67±0.06# | 50.6±0.8*** |
| D2 | 5.17±0.38 | 11.2±0.5 | 0.19±0.04 | 21.3±0.9*** | 10.2±0.2*** | 1.86±0.34 | 0.54±0.10# | 47.9±0.6* | |
| D3 | 5.40±0.07* | 14.2±0.2** | 0.21±0.03 | 21.5±0.2*** | 10.9±0.1*** | 2.20±0.42 | 0.62±0.01# | 39.0±1.1 | |
| D6 | 4.45±0.22 | 12.5±0.4 | 0.12±0.01 | 14.7±0.2 | 9.23±0.15* | 2.05±0.42 | 0.50±0.02# | 40.6±1.1 | |
| D9 | 4.39±0.38 | 16.7±1.1*** | 0.11±0.03 | 11.7±0.1** | 9.54±0.65** | 2.05±0.26 | 0.52±0.07# | 37.3±0.4* | |
| D12 | 4.87±0.43 | 18.7±0.9*** | 0.15±0.01 | 12.2±1.2* | 10.1±0.3*** | 1.62±0.36 | 0.99±0.10# | 35.4±0.1*** | |
| Recovery (R) | R1′ | 3.24±0.11 | 13.8±0.6** | 0.13±0.02 | 18.9±0.9* | 9.47±0.12** | 1.02±0.05 | 0.53±0.07 | 38.6±1.2 |
| vs D3 | *** | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. | |
| R1 | 4.04±0.50 | 17.3±0.8*** | 0.12±0.03 | 17.3±0.5 | 9.49±0.19** | 0.89±0.17 | 0.84±0.23# | 35.5±1.0*** | |
| vs D6 | N.S. | ** | N.S. | N.S. | N.S. | N.S. | N.S. | * | |
| R2 | 4.54±0.15 | 15.9±1.1*** | 0.11±0.04 | 21.2±0.2** | 8.69±0.05 | 0.81±0.10 | 0.61±0.06# | 39.5±0.6 | |
| vs D6 | N.S. | N.S. | N.S. | *** | N.S. | N.S. | N.S. | N.S. | |
Macronutrient composition in hemolymph and body of flies kept for 1–12 weeks at 11°C and 10L:14D, light/dark (D1–D12) and after recovery for 1 week after 3 weeks of diapause (R1′), or 1 (R1) or 2 weeks (R2) after 6 weeks of diapause. Virgin flies, kept for 1 week at non-diapausing conditions (C1) and recently hatched 3–6 h old flies (C0) were used as a controls. Data are presented as means ± S.E.M, n = 3–5 independent replicates with 10–15 flies in every replicate. Significantly different either from the control (C1) with *p<0.05, ** p<0.01, *** p<0.001 or from the indicated group as assessed by ANOVA, followed by Tukey test or alternatively with # p<0.05, ## p<0.01, ### p<0.001 as assessed by Kruskal-Wallis test followed with Wilcoxon pairwise comparison. N.S. – values are not significantly different. Vs, versus.